Fig. 8 | International Journal of Oral Science

Fig. 8

From: Integrative single-cell and bulk transcriptomes analyses reveals heterogeneity of serine-glycine-one-carbon metabolism with distinct prognoses and therapeutic vulnerabilities in HNSCC

Fig. 8

Knockdown IMPDH1 represses HNSCC cell progression in vitro. a After treating with DMSO or guanosine (100 μmol/L), the cell viability of HSC6 IMPDH1shs cells were determined using CCK-8. The cell migration ablility was determined by Would healing assay (b) and the invasion ability was detected by Transwell assay with matrigel (c). The protein levels of endogenous apoptosis markers and nucleolar stress markers were measured by western blot (d). The apoptosis was tested using flow cytometry (e). f LC was performed to measure the relative GTP contents in HSC6 IMPDH1shs cells treated with DMSO or guanosine. g Effects of IMPDH1 knockdown on nuclear size and morphology of HNSCC cells. Mean ± s.d.; *P < 0.05; two-way ANOVA. Scale bars, (b, c) 100 μm, (g) 5 μm

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