Fig. 2 | Laboratory Investigation

Fig. 2

From: Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-κB signaling pathway

Fig. 2

Upregulation of Fezf2 inhibits bladder cancer cell aggressiveness in vitro. a Western blotting analysis of Fezf2 expression in EJ and T24 cells stably expressing Fezf2; α-tubulin was used as a loading control. b Representative micrographs (left panel) and quantification (right panel) of EdU incorporation in indicated bladder cancer cells. DAPI was used as a DNA/nuclear stain. c Representative images of CAM blood vessels stimulated with conditioned medium from indicated cells. d Flow cytometric analysis showing the percentages of cells overexpressing Fezf2 at different phases of the cell cycle. e Representative pictures (left panel) and quantification (right panel) of invading cells were analyzed using a transwell Matrigel assay. f Representative images (left panel) and quantification (right panel) of HUVECs cultured on matrigel-coated plates with conditioned medium from cells overexpressing Fezf2. Bar graphs show statistical analysis of three independent experiments (*P < 0.05)

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