Fig. 4
Exogenous expression of Notch ligands Dll11 and Jag1 enhances BMP9-induced osteogenic differentiation of MSCs. a Adenovirus-mediated overexpression of Notch ligands Dll1 and Jag1. Subconfluent C3H10T1/2 cells were infected with AdR-Dll1, AdR-Jag1, or AdRFP control virus for 36 h. Total RNA was collected and subjected to sqPCR analysis using primers for mouse Dll1, Jag1, or Gapdh. The sqPCR was done in triplicate and representative results are shown. b Overexpression of Dll1 or Jag1 enhances BMP9-induced ALP activity in MSCs. Subconfluent C3H10T1/2 cells were co-infected with AdBMP9 or AdGFP and AdR-Dll1 (a) or AdR-Jag1 (b). At 3, 5, and 7 days after infection, the ALP activity of the infected cells were determined by quantitative chemiluminescence assay. Each assay condition was done in triplicate. Representative results are shown. *p < 0.05, **p < 0.001. c Overexpression of Dll1 or Jag1 augments BMP9-induced matrix mineralization. Subconfluent C3H10T1/2 cells were co-infected with AdBMP9 or AdGFP and/or AdR-Dll1 (a) or AdR-Jag1 (b) and maintained in the mineralization medium for 14 days. The cells were fixed and subjected to Alizarin Red S staining. Each assay condition was done in triplicate. Representative results are shown
