Fig. 1: The role of HBc in NEU1 expression in HBV-related hepatoma cells.

a The role of HBV in the expression of human sialidases in hepatoma cells at the mRNA level detected with real-time PCR. b The expression of NEU1 protein mediated by HBV measured via western blot analysis. c Bioinformatics analysis of NEU1 expression in HBV-related HCC tissues and non-HCC tissues using GSE14520 microarray data. d The expression of NEU1 protein in HBV-negative adjacent tissues (n = 10), HBV-negative HCC tissues (n = 30), HBV-positive adjacent tissues (n = 30), and HBV-related tumor tissues (n = 50) was assessed via IHC analysis (×200). e The role of encoding genes in the HBV genome in NEU1 mRNA expression in hepatoma cells. f The role of encoding genes in the HBV genome in NEU1 protein expression in HepG2 and Huh7 cells. g The role of HBV and HBc in NEU1 mRNA expression in L02 cells. h The role of HBV and HBc in NEU1 protein expression in L02 cells. i The expression of NEU1 mRNA in HBc stably transfected hepatoma cells. j The expression of NEU1 protein in HBc-positive hepatoma cells. k The expression of NEU1 and HBc proteins in HBV-related tumor tissues assessed via IHC analysis (×200). l The expression of NEU1 in HBc-positive cells and control cells assessed with immunofluorescence assays. m Sialidase activity in HBc-positive cells and control cells. HepG2-NC, Huh7-NC: HepG2 and Huh7 cells transfected with control plasmids. HepG2-HBV, Huh7-HBV: HepG2 and Huh7 cells transfected with HBV plasmids. HepG2-Mock, Huh7-Mock: HepG2 and Huh7 cells transfected with control plasmids. HepG2-HBc, Huh7-HBc: HepG2 and Huh7 cells transfected with HBc plasmids. *P < 0.05.