Fig. 2: The effect of the NF-κB pathway in activation of the NEU1 promoter mediated by HBc in hepatoma cells. | Laboratory Investigation

Fig. 2: The effect of the NF-κB pathway in activation of the NEU1 promoter mediated by HBc in hepatoma cells.

From: Hepatitis B virus core protein promotes the expression of neuraminidase 1 to facilitate hepatocarcinogenesis

Fig. 2

a Identification of the regulatory region of the NEU1 promoter influenced by HBc via a luciferase reporter gene assay. b Information regarding the NF-κB binding site in the core region of the NEU1 promoter and associated mutations in the PGL3-P (−1398/+100) plasmid. c The activation of P65 in HBc-positive cells and control cells. d The expression of IkBα in HBc-positive cells and control cells. e The ___location of P65 in HBc-positive cells and control cells. f The influence of mutation in the NF-κB binding site on activation of the core region of the NEU1 promoter in the PGL3-P (−1398/+100) plasmid. g The effect of inhibition of NF-κB via BAY11-7082 on the activity of the core region of the NEU1 promoter in the PGL3-P (−1398/+100) plasmid in HBc-positive hepatoma cells. h The effect of P65 inhibition using shRNA plasmids on the expression of P65 protein in HBc-positive hepatoma cells. i The effect of P65 inhibition via shRNA plasmids on the activity of the core region of the NEU1 promoter in PGL3-P (−1398/+100) plasmid in HBc-positive hepatoma cells. j The effect of P65 inhibition using shRNA plasmids on NEU1 protein expression in hepatoma cells. k The effect of P65 inhibition via BAY11-7082 on NEU1 protein expression in hepatoma cells. *P < 0.05.

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