Fig. 6: IL-37b suppressed the migration and invasion ability of endometrial cancer cells by the Rac1/NF-κB/MMP2 signal pathway. | Laboratory Investigation

Fig. 6: IL-37b suppressed the migration and invasion ability of endometrial cancer cells by the Rac1/NF-κB/MMP2 signal pathway.

From: IL-37bΔ1-45 suppresses the migration and invasion of endometrial cancer cells by targeting the Rac1/NF-κB/MMP2 signal pathway

Fig. 6

A Ishikawa cells infected with IL-37bΔ1-45 lentivirus (LV-IL-37bΔ1-45) showed higher expression of IL-37 than that infected with negative control lentivirus (LV-NC) detected by qPCR and western blot (p < 0.0001). The expression of MMP2 protein was downregulated in Ishikawa cells with IL-37bΔ1-45 lentivirus detected by western blot. LV-IL-37bΔ1-45 decreased significantly the number of cells migrating (B) or invading (C) the lower surface of chambers compared with the LV-NC group (p < 0.05). D The inhibitory effect of IL-37bΔ1-45 on MMP2 and p-P65 was reversed after the Rac1-61L overexpression plasmid was used to transfect LV-IL-37bΔ1-45 stably expressed cells (ISK-LV-IL-37bΔ1-45). Rac1-61L overexpression significantly increased the number of IL-37bΔ1-45 stably expressed cells migrating (E) or invading (F) the lower surface of chambers compared with the MOCK group (PRK5 control plasmid) (p < 0.05). LV Lentivirus. *p < 0.05; **p < 0.01; ***p < 0.001.

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