Fig. 2: The effect of lncRNA SNHG15 overexpression on proliferation, migration, tube formation, and apoptosis of HG-treated HUVECs. | Laboratory Investigation

Fig. 2: The effect of lncRNA SNHG15 overexpression on proliferation, migration, tube formation, and apoptosis of HG-treated HUVECs.

From: LncRNA SNHG15 relieves hyperglycemia-induced endothelial dysfunction via increased ubiquitination of thioredoxin-interacting protein

Fig. 2

HUVECs were divided into four groups: NG, HG, HG+ lentivirus (Lenti)-SNHG15, and HG+ the negative control of Lenti-SNHG15 (Lenti-NC). A SNHG15 expression was measured by qRT-PCR. B Cell proliferation was detected by cell counting kit-8 (CCK-8) assay. C Cell migration was measured by the transwell assay. D Representative images of tube formation assay performed on HUVECs were shown (scale bar = 400 µm). The tube length and branch points were measured utilizing Image-Pro Plus software. E Cell apoptosis was measured using flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001 vs NG; #P < 0.05, ##P < 0.01, ###P < 0.001 vs HG + Lenti-NC.

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