Fig. 3: Nitric oxide (NO)-producing hydrogel PA-YK-NO worsens response to hindlimb ischemia in sickle cell mice. | Laboratory Investigation

Fig. 3: Nitric oxide (NO)-producing hydrogel PA-YK-NO worsens response to hindlimb ischemia in sickle cell mice.

From: Increasing nitric oxide bioavailability fails to improve collateral vessel formation in humanized sickle cell mice

Fig. 3

A Serum nitrate/nitrite (NOx) in sickle cell (SS) mice treated with vehicle or PA-YK-NO (0.9 µM NO) and subjected to hindlimb ischemia (HLI) surgery at day 14 post surgery, n = 4–5, *P < 0.05, student’s unpaired t-test. B Whole limb and (C) lower limb perfusion recovery at day 1, day 7 and day 14 after HLI in SS mice treated with vehicle or PA-YK-NO. Representative LDPI images shown on RHS, whole limb area indicated with red box, lower limb area indicated with yellow box, n = 4–5, *P < 0.05, **P < 0.01, ****P < 0.0001, 2-way Repeated Measures ANOVA, Sidak’s post hoc test. D HLI Day 14 necrosis score. E Ischemic limb peroxynitrite (ONOO-). F Ischemic limb hydrogen peroxide (H2O2) in SS mice treated with vehicle or PA-YK-NO, n = 4–5, *P < 0.05, student’s unpaired t-test. Results expressed as mean ± SEM.

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