Fig. 4: L-NG-nitro arginine methyl ester (L-NAME) worsens response to hindlimb ischemia but protects against aortic inflammation in sickle cell mice. | Laboratory Investigation

Fig. 4: L-NG-nitro arginine methyl ester (L-NAME) worsens response to hindlimb ischemia but protects against aortic inflammation in sickle cell mice.

From: Increasing nitric oxide bioavailability fails to improve collateral vessel formation in humanized sickle cell mice

Fig. 4

A Serum nitrate/nitrite (NOx), (B) Necrosis score and (C) Lectin positive staining (% area) in ischemic hindlimb in sickle cell (SS) mice treated with L-NAME (1 g/L in drinking water) at day 7 post hindlimb ischemia surgery, n = 5 or n = 10, *P < 0.05, ***P < 0.001, students unpaired t-test. LHS shows representative confocal microscopy image of fluorescein isothiocyanate lectin-perfused vessels showing significantly decreased vessel formation in L-NAME treated mice (scale bar = 20 µm). Aortic mRNA expression of proinflammatory cytokines (D) interleukin 6 (IL-6) and (E) tumour necrosis factor α (TNFα) in wildtype (AA) and sickle cell (SS) mice treated with L-NAME for 7 days, n = 4–6, *P < 0.05, ***P < 0.001, One-way ANOVA, Sidak’s post hoc test. Results expressed as mean ± SEM.

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