Fig. 1: Resistant cells (NCI-H1299R) are more resistant to radiation than parental cells (NCI-H1299). | Laboratory Investigation

Fig. 1: Resistant cells (NCI-H1299R) are more resistant to radiation than parental cells (NCI-H1299).

From: E3 ubiquitin ligase FBXW7 enhances radiosensitivity of non-small cell lung cancer cells by inhibiting SOX9 regulation of CDKN1A through ubiquitination

Fig. 1

A NCI-H1299 and NCI-H1299R cells were irradiated with 0, 2, 4, and 6 Gy dose of X-ray for 14 days, respectively, and then the cell survival was detected using colony formation assay. B, C NCI-H1299 and NCI-H1299R cells were further irradiated with 6 Gy dose of X-ray, and the proliferation and apoptosis were detected using CCK-8 assay and flow cytometry. D γ-H2AX was detected using immunofluorescence, scale bars: 10 μm. The cell experiment was conducted 3 times independently. Data were presented as mean ± standard deviation. Data in A/B were analyzed using two-way ANOVA, and data in C/D were analyzed using t test, followed by Tukey’s multiple comparison test, *p < 0.05.

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