Fig. 3: FBXW7 interacted with SOX9 and promoted ubiquitination and proteasome degradation of SOX9. | Laboratory Investigation

Fig. 3: FBXW7 interacted with SOX9 and promoted ubiquitination and proteasome degradation of SOX9.

From: E3 ubiquitin ligase FBXW7 enhances radiosensitivity of non-small cell lung cancer cells by inhibiting SOX9 regulation of CDKN1A through ubiquitination

Fig. 3

A, B GEPIA website (http://gepia.cancer-pku.cn/detail.php?gene=SOX9) showed that SOX9 was highly expressed in LC (including squamous cell carcinoma and adenocarcinoma) and associated with poor prognosis. In the figure, red is the tumor group and gray is the normal group. C, D NCI-H1299 and NCI-H1299R cells were transfected with oe-NC and oe-FBXW7, and then SOX9 expression was detected using qRT-PCR and western blot. E The interaction between FBXW7 and SOX9 was detected by immunoprecipitation assay in NCI-H1299 cells. F Ubiquitin pull-down assay was used to detect the ubiquitin level of SOX9 after transfection of oe-NC and oe-FBXW7 into NCI-H1299 cells. G The transfected NCI-H1299 cells were treated with CHX (10 μM) for 0, 8, 16, and 24 h to inhibit protein synthesis, and then SOX9 expression was detected using western blot. H The transfected NCI-H1299 cells were treated with MG132 (20 μM) for 8 h, and then SOX9 expression was detected using western blot. The cell experiment was conducted 3 times independently. Data were presented as mean ± standard deviation and analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test, *p < 0.05.

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