Fig. 5: FBXW7 promoted CDKN1A expression via SOX9. | Laboratory Investigation

Fig. 5: FBXW7 promoted CDKN1A expression via SOX9.

From: E3 ubiquitin ligase FBXW7 enhances radiosensitivity of non-small cell lung cancer cells by inhibiting SOX9 regulation of CDKN1A through ubiquitination

Fig. 5

A, B GEPIA website (http://gepia.cancer-pku.cn/detail.php?gene=SOX9) showed that CDKN1A was highly expressed in LC (including squamous cell carcinoma and adenocarcinoma) and associated with poor prognosis. In the figure, red is the tumor group and gray is the normal group. C CDKN1A expression in NCI-H1299R cells was detected using qRT-PCR. D JASPAR website (http://jaspar.genereg.net/analysis) predicted that SOX9 had a binding site in the promoter of CDKN1A. E, F Dual-luciferase reporter gene assay and ChIP assay verified that SOX9 could bind to the promoter of CDKN1A. The cell experiment was conducted 3 times independently. Data were presented as mean ± standard deviation. Data in C were analyzed using one-way ANOVA, data in E were analyzed using two-way ANOVA, and data in F were analyzed using t test, followed by Tukey’s multiple comparison test, *p < 0.05.

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