Fig. 3: Extracellular ATP metabolism through CD39 and CD73 expressed on HTLV-1 infected cell lines and patients’ leukemic cells. | Leukemia

Fig. 3: Extracellular ATP metabolism through CD39 and CD73 expressed on HTLV-1 infected cell lines and patients’ leukemic cells.

From: Ectonucleotidase CD39 is highly expressed on ATLL cells and is responsible for their immunosuppressive function

Fig. 3

a The experimental scheme. Cell lines and patients’ leukemic cells were cultured for 1 or 3 h in 10% FCS RPMI supplemented with 1 mM ATP, and then supernatants were subjected to ATP or AMP/adenosine measurement. b Contour plots in the upper panel show CD39 and CD73 expression on T-cell lines. Flow cytometry was used to examine the expressions of CD39 and CD73 on the Jurkat cell line, human T-cell lymphoma (other than ATLL) cell line, and the MJ, M1, M2, and M4 HTLV-1 infected cell lines. Bar graph in the lower panel shows the remaining extracellular ATP concentration after culture for the indicated hours. Bar heights indicate the mean ± SD of the % ratio of remaining ATP to the supplemented amount from triplicate experiments. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test, compared with the value of Jurkat. c The same experiments as in b using primary CD4+ T cells. Lower panel shows relative remaining ATP concentrations after 1 h of incubation. Data significance was evaluated compared with the value of the normal CD4+ T cells. d AMP concentrations after 1 h (black) or 3 h (white) of incubation are shown. e Adenosine concentrations after the culture of CD39-expressing and/or CD73-expressing ATLL leukemic cells. Leukemic cells from Case 32 express CD39 but not CD73, and those from Cases 12 and 23 express both CD39 and CD73.

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