Fig. 5: PV macrophages are more pro-phagocytic. | Leukemia

Fig. 5: PV macrophages are more pro-phagocytic.

From: Blocking the CD47-SIRPα interaction reverses the disease phenotype in a polycythemia vera mouse model

Fig. 5

A Experimental workflow for the in vitro phagocytosis assay of wild-type (WT) and JAK2 mutant (PV) macrophages with WT or PV RBCs treated with an anti-IgG1 control (IgG1) or an anti-CD47 antibody. Created with BioRender.com. Percentage of labeled WT RBCs (B) and PV RBCs (C) out of F4/80+ spleen macrophages from WT and PV mice either treated with IgG1 or anti-CD47. Representative flow cytometry plots are shown on the left. D Percentage of labeled WT RBCs and JAK2 mutant RBCs (PV RBCs) out of WT (left) and PV (right) F4/80+ spleen macrophages either treated with IgG1 or anti-CD47. WT, blue symbols; PV, red symbols. Results are represented as mean ± standard deviation. ns not significant, *p < 0.05, **p < 0.01, ****p < 0.0001 (two-way ANOVA with Tukey’s multiple comparisons test for B–D).

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