Fig. 7: Chemical resolution recovery by the RSPSSL scheme.

a Representative preprocessed Raman spectral image of the Hela cell line mapped with Raman shifts of the molecular fingerprint region. b Raman shift indication on the average spectra of the corresponding Raman spectral image. Each blue spectrum demonstrates the average of 10 000 spectra with top SNR_spec of the raw data image. Each red spectrum shows the average of 10 000 RSBPCNN^# preprocessed spectra with top SNR_spec from the corresponding hyperspectral image. c Average 10 000 spectra with top SNR_spec of each image preprocessed by different methods. d Merged images of the Raw data or RSBPCNN^# preprocessed spectra with Raman shifts of 733 cm⁻¹ (red, phosphatidylserine), 1200 cm⁻¹ (blue, nucleic acids & phosphates/C-O/C-N), and 1224 cm⁻¹ (green, amide III (β-sheet structure)). Scale bar: 20 μm