Fig. 1: Use of the vacuum-assisted micropore array for multiplexed single-cell patterning.
From: On-chip multiplexed single-cell patterning and controllable intracellular delivery
a A cross-sectional view of the 3D low-voltage electroporation (3D EP) platform setup. b A diagram of the pyramid pit-shaped micropore array chip. The zoomed-in view showed a single cell loaded on a micropore. c Photograph of the 3D EP platform. Scale bar: 1 cm. d Scanning electron microscopy showed both a pyramid pit array (top side) and a micropore array (back side). Scale bar: left 100 µm, right 20 µm. e Vacuum-assisted multiplexed single-cell array pattern on the chip. Scale bar: 100 µm. f The cell capture rate obtained with different ratios of the loaded cell number to the micropore number at a fixed pressure (1.5 psi). g 3D single-cell fluorescence model, as revealed by confocal microscopy, showing the percentage of the cell body inside the micropore (5 µm) after cell trapping. Scale bar: 10 µm.
