Fig. 5: DE genes associated with Li treatment in responders show enrichment for spliceosome and OXPHOS pathways. | Molecular Psychiatry

Fig. 5: DE genes associated with Li treatment in responders show enrichment for spliceosome and OXPHOS pathways.

From: Lithium increases mitochondrial respiration in iPSC-derived neural precursor cells from lithium responders

Fig. 5

a Venn diagram of DE genes associated with Li response status after Li treatment for 6 h, showing shared DE genes (464) and unique DE genes for Li-N (1059) and Li-R (554) NPCs. b Volcano plot of Li-R unique DE genes (p value < 0.05). c Pathway (KEGG) enrichment analysis of Li-R unique DE genes (554) showing significant enrichment for spliceosome, oxidative phosphorylation (OXPHOS) and thermogenesis pathways. d Initial analysis of overall intron retention rate (IRR), measured as the percentage of sequencing reads mapping to intronic regions, in NPCs after 6 h and 1 week treatment with DMSO, Li, VPA and LTG. 6 h of exposure led to significantly lower levels of intron retention for all treatments, most pronounced for Li. No comparable effect was found for 1 week exposure. F statistic and p value of repeated measures ANOVA tests are shown. Plots displaying transcriptome-wide per-intron IRR by chromosome ___location for 6 h treatment with Li (e), VPA (f) and LTG (g). Colored dots represent introns with significant (IR change >20% and FDR < 0.05) differences in retention rate in drug-treated samples compared to DMSO samples.

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