Fig. 2: Characterization of Li treated hCS. | Molecular Psychiatry

Fig. 2: Characterization of Li treated hCS.

From: Transcriptional and functional effects of lithium in bipolar disorder iPSC-derived cortical spheroids

Fig. 2

a Representative images of CTRL and BD hCS from both Li-N and Li-R patients at days 30, 90 and 150 of differentiation. b Growth of CTRL and BD hCS size through differentiation show decreased volume at most time points of Li-N and Li-R hCS compared to CTRL hCS. Individual hCS volumes were estimated from their areas measured with ImageJ. c Li induced growth after 1 month treatment from day 150 until day 180. Both CTRL and Li-R, but not for Li-N hCS, present a significant increase in size. d Representative immunohistochemistry images of CTRL, Li-N and Li-R hCS with and without Li treatment. Fluorescence was measured for cytoplasmic MAP2 (e) and SV2A (f), by counting signal intensity with ImageJ. Results are presented in arbitrary units (a.u.). CTRL (N = 10), Li-N (N = 5), Li-R (N = 6). Data was analyzed by Mann–Whitney U test and Wilcoxon test for unpaired and paired group comparisons respectively. *p < 0.05, **p < 0.01, ***p < 0.001.

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