Fig. 4: Effect of TLR9 p.E317D variant on signaling. | Molecular Psychiatry

Fig. 4: Effect of TLR9 p.E317D variant on signaling.

From: Mutated Toll-like receptor 9 increases Alzheimer’s disease risk by compromising innate immunity protection

Fig. 4

HEK293 cells transiently transfected with a plasmid containing TLR9 wild type (A) or mutated (B–E) and a destabilized form of NanoLuc luciferase reporter driven by an NF-κB response element. TLR9 activation was achieved with different concentrations of the CpG ODN2006 or 0.1 ng/µl TNF-α as control for expression levels. Luminescence is expressed relative to the TNF-α response in the same cell line with the TLR9 wild type response set at 100%. The average of 3 independent experiments is shown. In the table, logarithmic scale of the half maximal effective concentration (pEC50) and maximum responses (% Effect) are reported.

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