Fig. 1

CRYAB is decreased in the inflamed colon tissues from IBD patients. a qRT-PCR analysis of CRYAB, TNF-α, and IL-6 expression. RNA was isolated from healthy controls (HC, n = 45), active UC patients (A-UC, n = 30), and active CD patients (A-CD, n = 33). CRYAB was significantly decreased, whereas TNF-α and IL-6 were markedly increased in A-UC and A-CD than those in HC, (*p < 0.05, ***p < 0.001). b Relationships between TNF-α, IL-6, and CRYAB mRNA in inflamed mucosa from 33 A-CD. Significant correlations existed between the levels of TNF-α mRNA and CRYAB (Spearman’s rank correlation coefficient, R = −0.4744, p = 0.0061), and IL-6 and CRYAB expression (Spearman’s rank correlation coefficient, R = −0.4631, p = 0.0067). c The expression of CRYAB, p65, p-p65, IκBα, and p-IκBα in the inflamed and unaffected tissues from six paired A-UC and six paired A-CD patients was examined by immunoblotting with specific antibodies. d Representative immunohistochemical images of CRYAB, CD68, CD3, and CD4 in the paired, inflamed, and unaffected tissues of the same CD patients (magnification: ×200, upper panels; ×400, lower panels). All data are shown from three independent experiments