Fig. 2: Integrin β7-deficient IL-10−/− mice display deficits in colonic B cells populations, fecal immunoglobulin levels, and develop more severe colitis. | Mucosal Immunology

Fig. 2: Integrin β7-deficient IL-10−/− mice display deficits in colonic B cells populations, fecal immunoglobulin levels, and develop more severe colitis.

From: Antibody secreting cells are critically dependent on integrin α4β7/MAdCAM-1 for intestinal recruitment and control of the microbiota during chronic colitis

Fig. 2

a Weights, rectal temperatures and b survival curves of indicated strains. c Colonic and cecal histological indices and representative colonic pathology. d Colonic LP CD3+ T-cell and e CD19+ B-cell counts of indicated strains at 8–12 weeks of age, determined by flow cytometry (noninflamed (N/I, histological index ≤ 1), inflamed (I histological index > 3), freshly isolated LP cells were stained and events gated on live single cells. f Representative flow cytometry plots of colonic LP IgM+ and IgA+ cells. Events were gated on live, single, CD3−, CD19− cells. g Colonic LP IgM+ and IgA+ cell counts of indicated strains. h Representative confocal immunofluorescence images demonstrating immunolocalization of colonic IgA+ ASC in the indicated mouse strains. i Fecal IgM and IgA of indicated mouse strains. Expression was normalized to total fecal protein. All data are presented as mean ± S.D, from n > 9 mice in each dataset. Each data point represents an individual mouse. Statistical significance was determined by ANOVA, followed by Tukey’s multiple comparison test (a, b, d, e) or Student’s t test (g–i).

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