Fig. 4: MAdCAM-1 blockade altered the percentage of B cell/ASC in colon and blood, reversed fecal, and plasma IgA levels, hastened colitis, and induced bacterial overgrowth and changes in microbiota composition. | Mucosal Immunology

Fig. 4: MAdCAM-1 blockade altered the percentage of B cell/ASC in colon and blood, reversed fecal, and plasma IgA levels, hastened colitis, and induced bacterial overgrowth and changes in microbiota composition.

From: Antibody secreting cells are critically dependent on integrin α4β7/MAdCAM-1 for intestinal recruitment and control of the microbiota during chronic colitis

Fig. 4

In IL-10−/− mice, a mass cytometry opt-SNE analysis of concatenated colonic LPMC isolated from mice undergoing the indicated treatments, pregated on nucleated, live, CD45+ events. b Percentages of the indicated cell lineages within LPMC fractions of mice treated with MECA-367 or isotype control. c Serial rectal temperatures and d serial weights. e Colonic and cecal histological indices of treated mice at week 8. f Serial fecal IgA and g plasma IgA levels of treated IL-10−/− mice. h Serial peripheral blood cell counts of IgA+ cells. i Fecal bacterial counts from IL-10−/− mice treated with isotype or MECA-367, determined via RT-qPCR for 16S rRNA expression. j Alpha diversity (Shannon’s index) and k beta diversity principal coordinate analysis and of IL-10−/− mice receiving indicated antibodies. All data are presented as mean ± S.D, from n > 10 mice in each dataset. Statistical significance was determined by Student’s t test (b) or two-way ANOVA, followed by Sidak’s multiple comparison test.

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