Fig. 3: Impact of neonatal LTβR signaling on the expression of eosinophil chemokine genes in MLN stromal cells from RV-infected adult mice.

a RNA-seq data analysis of indicated genes in WT MLN stromal cells (n = 3 mice per group) at d8 post-RV infection. Two independent experiments were performed, and one representative experiment is shown. b qPCR measurement of Ccl11, Ccl6, Ccl7, Ccl9, and Cxcl2 from MLN stromal cells derived from d8 RV-infected WT mice that were treated with LTβR-Ig versus control Ig at postnatal d2–d16 in an independent cohort of mice. Data were combined from two independent experiments (n = 4 samples per experiment; each sample was pooled from two mice). Data are presented as mean ± SEM and were analyzed using two-tailed unpaired Mann–Whitney test.