Fig. 5: Restoration of eosinophil deficiency in the MLN of LTβ^−/− chimeric mice does not rescue the defective anti-RV IgA responses.

a Representative FACS plots of MLN Siglec-F⁺CD11b⁺ cells from Blimp-1^YFP→WT, Blimp-1^YFP→LTβ^−/− and IL-5/Blimp-1^YFP→LTβ^−/− chimeric mice at d8 post-RV infection. b Frequency of MLN Siglec-F⁺CD11b⁺ cells from Blimp-1^YFP→WT, Blimp-1^YFP→LTβ^−/− and IL-5/Blimp-1^YFP→LTβ^−/− chimeric mice at d8 post-infection. Y-axis shows the frequency of Siglec-F⁺CD11b⁺ cells among live CD45⁺/CD3⁻/B220⁻/FcεRI⁻/CD11c⁻/Ly6G^low MLN cells. c Frequency of YFP^high B cells in the MLN of Blimp-1^YFP→WT, Blimp-1^YFP→LTβ^−/− and IL-5/Blimp-1^YFP→LTβ^−/− chimeric uninfected mice and chimeric mice at d8 post-RV infection, respectively. d Enumeration of RV-specific IgA-ASC in the MLN (left panel) and SILP (right panel) of Blimp-1^YFP→WT, Blimp-1^YFP→LTβ^−/− and IL-5/Blimp-1^YFP→LTβ^−/− chimeric mice at d8 post-infection. Data in (b–d) were presented as mean ± SEM and analyzed using two-tailed unpaired Mann–Whitney test.