Fig. 1: The effects of metformin on serum TMA and TMAO levels in C57BL/6J mice under chow or choline diet. | Nutrition & Diabetes

Fig. 1: The effects of metformin on serum TMA and TMAO levels in C57BL/6J mice under chow or choline diet.

From: Metformin alleviates choline diet-induced TMAO elevation in C57BL/6J mice by influencing gut-microbiota composition and functionality

Fig. 1

a, b Female C57BL/6J mice were fed chow with or without 100 mg/kg metformin (Met-100) or 200 mg/kg metformin (Met-200) for 16 days, then fed 1% choline diet with or without metformin (veh (c), Met-100 (c), Met-200 (c)) for another 9 days. Mice administration as illustrated in schematic diagram (a). TMA and TMAO levels were detected at indicated time points by HPLC-MS/MS (b). veh/veh (c), n = 5; chow, n = 5; Met-100/Met-100 (c), n = 8; Met-200/Met-200 (c), n = 8. c 2% choline fed C57BL/6J mice were applied to evaluate the effects of metformin on TMA and TMAO levels. C57BL/6J mice were initially fed on chow diet with or without 200 mg/kg metformin for 6 weeks and sequentially converted to 2% choline diet for another 3 weeks. Serum TMA and TMAO levels were determined. n = 5 for each group. Values are presented as means ± SEM (*p < 0.05, ***p < 0.001).

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