Fig. 2: Metformin treatment altered composition of gut microbiota and inhibited TMA production from choline. | Nutrition & Diabetes

Fig. 2: Metformin treatment altered composition of gut microbiota and inhibited TMA production from choline.

From: Metformin alleviates choline diet-induced TMAO elevation in C57BL/6J mice by influencing gut-microbiota composition and functionality

Fig. 2

a Effects of metformin on enterotype of C57BL/6J mice fed with chow or choline diet. Samples were calculated using Jensen-Shannon distance and separated into two clusters by the PAM method. The left panel showed the clustering of two top principal components. b Linear regression analysis of the correlation between serum TMAO level and microbiota beta diversity based on OTU levels with bray curtis algorithm. c The abundance of significant different genera among four groups. Metformin, 200 mg/kg. Boxes show the median with interquartile ranges, whiskers show the minimum and maximum values. p-values are from Kruskal–Wallis H test (*p < 0.05, **p < 0.01, ***p < 0.001). d The abundances of cutC gene in fecal samples were measured by qPCR. veh/veh (c), n = 5; chow, n = 5; Met-100/Met-100 (c), n = 8; Met-200/Met-200 (c), n = 8. The error bars represented the mean ± SEM (*p < 0.05, ***p < 0.001). e Effects of metformin on TMA-producing bacteria under anaerobic condition. The production of d9-TMA from d9-choline (bar) and OD600 (line) were measured after metformin treatment for 24 h.

Back to article page