Fig. 2

NDRG2 dictates intestinal epithelial cell differentiation. a Immunofluorescent staining of E-cadherin, Ndrg2, p21, and p27 in intestinal epithelium from C57BL/6 mice at the age of 1, 2, or 3 months as indicated. b Quantification of fluorescence intensity ImageJ. c AKP activities of intestinal epithelial cells from mice at the indicated age. d Schematic representation of the Ndrg2 targeting vector (top), wild-type locus (WT, middle), targeted allele (Ndrg2-floxed, middle), and null allele (Ndrg2-Null, bottom). Exons are represented by solid rectangles. The targeting vector, which is flanked by 3675 bp of 5′ homology and 2138 bp of 3′ homology, contains a LoxP-FRT-Neo-FRT cassette and the second LoxP sequence in introns 1 and 6, respectively. e–g Immunofluorescent staining and intensity quanification of Ndrg2, p21, and p27, and AKP activity were measured in intestinal epithelium from 2-month WT or NDRG2-/- mice. *P < 0.05