Fig. 7

NDRG2 suppression of Skp2 controls cell differentiation. a-c We overexpressed wild-type Skp2 (wSkp2) and Skp2 S72A mutant (mSkp2) using relative expressing lentivirus in HT29 cells with or without NDRG2 expression. a Levels of the indicated proteins were determined in each cell population. b Cell viabilities in each group were determined by MTT assay. c AKP activity was determined in each cell population following a 48-h treatment of 2 mM NaBT. Data are expressed as means ±SD (n = 3). *P < 0.05. d, e HT29 cells were transfected with control or Skp2 siRNA. 24 h after transfection, cells were treated with 2 mM NaBT for additional 48 h. Indicated protein levels and AKP activity were determined relatively. Data are expressed as means ±SD (n = 3). *P < 0.05