Fig. 1

CBAP is highly expressed in T-ALL cell lines and patient cells, and it is important for the regulation of cell proliferation. a, b Immunoblots of CBAP in human peripheral CD3⁺ T lymphocytes and Jurkat cells (a), and in a panel of T-ALL and acute myeloid leukemia (AML) cell lines (b). c, d IHC staining with CBAP, CD3 and c-Myc antibodies was performed on bone marrow biopsy specimens from two T-ALL patients (T-ALL P01 and P02) (c) or two anemia patients (BM 01 and 02) (d). Scale bars, 10 μm. e Growth of leukemic Jurkat cells stably transduced with lentiviruses expressing control shRNA (sh-Ctrl) or CBAP shRNAs (sh-CBAP no. 1 or no. 2), or clones with (CBAP-KO) or without (cr-Ctrl) CBAP knockout using a CRISPR/Cas9-CBAP-targeting vector. Data are expressed as means ± SD (n = 4). f Cell-cycle distribution of leukemic Jurkat cells without knockout (cr-Ctrl) or with knockout of CBAP (CBAP-KO), measured by BrdU/7-AAD staining. Data are expressed as means ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001, according to two-tailed unpaired Student t tests