Fig. 1

Identification of GUCY1A1 and GUCY1B1 as novel ERG-regulated genes that are clinically associated with TMPRSS2-ERG fusion in PCa. a ERG-upregulated genes were identified using Affymetrix microarray analyzes (Human Genome U133 Plus 2.0 Array) on VCaP cells stably infected with lentiviral shRNA against non-target-control or ERG and subsequently treated with ethanol or 10 nM DHT for 24 h. These genes were further examined for clinical correlation with ERG expression using TCGA PCa dataset. Note: The stable cells were hormone-starved for 3 days (d) before the treatment. b The mRNA expression correlation analyzes on GUCY1B1 vs. ERG or GUCY1A1 vs. ERG using TCGA PCa dataset (N = 333) from cBioPortal. The Pearson and Spearman rank correlation coefficient were shown and (*) indicates the p-value < 0.05. c, d Co-expressed genes associated with (c) GUCY1B1 or d GUCY1A1. e Co-exist mutations associated with the expressions of both GUCY1B1/A1. f Box plots for GUCY1B1 or A1 expression in ERG fusion-positive PCa vs. ERG fusion-negative PCa samples