Fig. 4: Cigarette smoke suppresses LKB1 expression via promoter methylation.
From: Cigarette smoke-induced LKB1/AMPK pathway deficiency reduces EGFR TKI sensitivity in NSCLC
a The protein expression of LKB1 in tumor tissues from NSCLC patients was analyzed by IHC staining. Scale bars: 50 μm. b The statistical data of IHC staining showed the correlation between expression of LKB1 and cigarette smoking in patients with EGFRWT NSCLC. c Total lysates of H292 cells treated with various concentrations of CSE for 48 h followed by western blot analysis with indicated antibodies. d Analysis of mRNA expression of LKB1 from CSE- and B[α]P-treated H292 cells by RT-qPCR. e, f Total lysates of CSE and B[α]P H292 stable clones were subjected to ChIP-qPCR to identify the methylation status on the CpG island of LKB1 promoter by anti-MeCP2 antibody (e) or anti-5-MC antibody (f). Data are shown as mean±SEM from experiments performed in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001.
