Fig. 4: Dimerization of PLK1 in space and time during the cell cycle.
From: A dimerization-dependent mechanism regulates enzymatic activation and nuclear entry of PLK1
A HeLa-PLK1–3xMyc cells were synchronized at the G1/S boundary by a double thymidine arrest (dt), released into fresh medium, and harvested at the indicated times. The accumulation of key protein markers was used to determine the cell-cycle stages. The levels of the indicated proteins were analyzed by western blotting. B IPs using anti-Myc antibody from HeLa-PLK1–3xMyc cell lysates were blotted for PLK1, PLK1-pT210, Bora, and Aur-A. C HeLa-PLK1–3xMyc cells were synchronized at the G1/S boundary by dt treatment and released for 9 h. (Left) FACS analysis of the cell populations. (Middle) Cell lysates were fractionated, subjected to immunoblotting for PLK1, PLK1-pT210, Bora, Aur-A, pAur-A T288, pMyt1-T495, Myt1, Calnexin, and Histone H3. (Right) Fractionated lysates were subjected to anti-Myc co-IP and blotted for PLK1, PLK1-pT210, Bora, and Aur-A.(n = 3).
