Fig. 3: POU2F1 enhances glycolysis and PPP activity in colon cancer cells. | Oncogene

Fig. 3: POU2F1 enhances glycolysis and PPP activity in colon cancer cells.

From: The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity

Fig. 3

A Heatmap analysis of the 141 DEGs between POU2F1 silencing and control SW620 cells. B The GO analysis of 141 DEGs. C KEGG analysis of 141 DEGs. D Seahorse analysis of glycolytic activity in the indicated cells following treatment with glucose (10 mM), oligomycin (1.0 µM), 2-Deoxy-d-glucose (2-DG, 50 mM). E, F The levels of glucose consumption and lactate production in the indicated cells. G The levels of G6PD activity in the indicated cells. H, I The levels of intracellular NADP+ and NADPH and the ratios of NADP+/NADPH in the indicated cells. J Flow cytometry analysis of intracellular ROS levels. The levels of intracellular ROS in the control HCT116 cells were designated as 100%. K, L Representative images of 18F-FDG PET/CT scanning of the mice with SW620-sh-POU2F1 1# or control SW620-scramble tumors (n = 5 per group). The SUV values in individual mice were calculated. Data are representative images or expressed as the mean ± SD of each group of samples analyzed in triplicate from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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