Fig. 6: Hp virulence factor and acid stimulate NCX1/TRPC1 coupling in human GC cells.

Summary tracings of [Ca²⁺]_cyt time courses in response to extracellular 0 Na⁺ (A), NH₄Cl (5 mM) (C) and pH 4.5 (E) in the presence of extracellular 2 Ca²⁺ or 0 Ca²⁺ (left). Summary data showing the peaks of 0 Na⁺, NH₄Cl and pH 4.5-increased [Ca²⁺]_cyt signaling in SGC7901 cells (right) (A, n = 20 cells; C, n = 11 cells; E, n = 11 cells, ^****P < 0.0001). Summary tracings of [Ca²⁺]_cyt time courses in response to 0 Na⁺ (B), NH₄Cl (5 mM) (D) and pH 4.5 (F) in the presence of 2 Ca²⁺ or 2 Ca²⁺ plus KB-R7943 (KB-R, 30 μM) (left). Summary data showing the peaks of 0 Na⁺, NH₄Cl and pH 4.5-increased [Ca²⁺]_cyt signaling in SGC7901 cells (right) (B, n = 20 cells; D, n = 23 cells; F, n = 26 cells, ^****P < 0.0001). Summary tracings of [Ca²⁺]_cyt time courses in response to 0 Na⁺ in the presence of 2 Ca²⁺ or 5Ca²⁺ in CHO-NCX1 (G) and CHO-K1 (H) cells. I Summary data showing the peaks of 0 Na⁺-increased [Ca²⁺]_cyt signaling described as in G, H (G, n = 23 cells; H, n = 17 cells, ^****P < 0.0001). J Western blot analysis of NCX1 protein levels in CHO-NCX1 and CHO-K1 cells. Summary tracings of [Ca²⁺]_cyt time courses in response to 0 Na⁺ (K, L) and NH₄Cl (5 mM) (O, P) in the presence of 2 Ca²⁺ in NC (K, O) or shTRPC1 (L, P) of SGC7901 cells. Summary data showing the peaks of 0 Na⁺ (M) or NH₄Cl (Q)-increased [Ca²⁺]_cyt signaling described as in K, L, O, P (n = 11 cells, ^****P < 0.0001). N, R RT-PCR analysis of TRPC1 mRNA levels in NC and shTRPC1 of GC cells (n = 3, ^*P < 0.05, ^****P < 0.0001). Each one is 3 independent experiments with similar results.