Fig. 6: Vcam1 depletion severely impairs iCCA cell growth under pMF suppression. | Oncogene

Fig. 6: Vcam1 depletion severely impairs iCCA cell growth under pMF suppression.

From: Spatiotemporal regulation of cholangiocarcinoma growth and dissemination by peritumoral myofibroblasts in a Vcam1-dependent manner

Fig. 6

A GFP fluorescence images of the 2D monocultures of Vcam1Ctrl and Vcam1KO cells on Day 1, 3 and 5. B Cell number counts of Vcam1Ctrl and Vcam1KO cells in (A). Student t-test, P value, ***< 0.001, *<0.05. C Quantification of the viable Vcam1Ctrl and Vcam1KO cells cultured in (A). D GFP fluorescence images of the spheroid monocultures of the Vcam1Ctrl and Vcam1KO PPTR cells on Day 4 and 14. E GFP fluorescence images of the tumor-pMF cocultures of the Vcam1Ctrl and Vcam1KO PPTR cells on Day 4 and 14. F Quantitative comparison of the growth area of the tumor cell spheroids in their mono- and co-culture. Statistics in B, C & F: Student t-test. P value, ****<0.0001; ***<0.001, **<0.01; *<0.05.

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