Fig. 7: Vcam1 depletion abolished the metastatic growth of iCCA cells in vivo but promote the dissemination of individual tumor cells. | Oncogene

Fig. 7: Vcam1 depletion abolished the metastatic growth of iCCA cells in vivo but promote the dissemination of individual tumor cells.

From: Spatiotemporal regulation of cholangiocarcinoma growth and dissemination by peritumoral myofibroblasts in a Vcam1-dependent manner

Fig. 7

A Top four rows: gross GFP images of the livers and lungs from mice orthotopically transplanted with Vcam1Ctrl or Vcam1KO1 cells; bottom row: microscopic GFP images of the lungs from the Vcam1Ctrl or Vcam1KO1 groups showing the higher number of DTCs in the latter. B Quantification of the numbers of DTCs in the lungs in (A). C Top two rows: gross GFP images of the lungs from TVI mice injected with Vcam1Ctrl or Vcam1KO1 cells; bottom row: representative H&E images of the lungs from the Vcam1Ctrl and Vcam1KO1 groups. D Quantification of the numbers of the lung metastases in (C). E The indicated IHC on serial sections of the Vcam1Ctrl and Vcam1KO1 lung metastases. Note the complete loss of E-cadherin in the Vcam1KO tumor (d). Statistics in B & D: Student t-test. P value, ****< 0.0001; **< 0.01.

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