Fig. 8: Partial Vcam1 neutralization increases the number of small lung metastases in a TVI model.

A Transwell migration assay and quantification of the PPTR cells treated with IgG or Vcam1^Ab showing increased cell migration with Vcam1 blocking. Student t-test, P value <0.0001. Cells derived from two PPTR tumors were tested. B Flow cytometry of CellTracker proliferation assay of the PPTR tumor cells treated with IgG or Vcam1^Ab found no changes in cell proliferation with Vcam1 blocking. C Gross ZsG images of the lungs collected from the indicated TVI models three weeks post injection. Vcam1^Ab treatment led to more but smaller lung metastases. D Representative images of the whole-section GFP scans of the lungs from mice treated with IgG or Vcam1^Ab. E Quantification of the area of individual lung metastases from the TVI model shows smaller lung metastases in the Vcam1^Ab-treated mice. Student t-test, P value, ****<0.0001. F Histogram of the size distribution of the lung metastases from the TVI model shows the larger numbers of small lung metastases in the Vcam1^Ab-treated mice. G The indicated staining of the lung metastases from the IgG or Vcam1^Ab groups. Arrows: Vcam1⁺ tumor cells; asterisk: Vcam1^- DTCs. All images share the same 100 μm scale bar.