Fig. 6: SRPK1 promotes binding of β-catenin to the EGFR promoter region to increase mEGFR accumulation and phosphorylation. | Oncogene

Fig. 6: SRPK1 promotes binding of β-catenin to the EGFR promoter region to increase mEGFR accumulation and phosphorylation.

From: Serine-arginine protein kinase 1 (SRPK1) promotes EGFR-TKI resistance by enhancing GSK3β Ser9 autophosphorylation independent of its kinase activity in non-small-cell lung cancer

Fig. 6

A Western blot analysis of β-catenin expression in the nuclear fractions. B qRT-PCR analysis of EGFR mRNA expression; data represent the mean ± SD (n = 3). C Western blot analysis of the expression and phosphorylation of membrane EGFR; Na+/K+-ATPase was used as a loading control. D qRT-PCR analysis of EGFR mRNA expression. E Western blot analysis of mEGFR expression after LEF1 knockdown in SRPK1-transduced NCI-H1650 cells and SRPK1-silenced NCI-H1975 cells. F IC50 of gefitinib verified in LEF1-silenced cells. *P < 0.05, **P < 0.01, ***P < 0.001. G The LEF1 binding regions (Region#1–3) in the 2-kb upstream promoter region of EGFR predicted by the JASPAR website. H Schematic diagram of the luciferase reporter plasmids containing wild-type and mutant predicted LFF1 binding regions, and (I) Luciferase assay after transfection into 293T cells (with or without Wnt3a treatment). J ChIP-qPCR assays performed with anti-β-catenin antibody and primers across region#1 in SRPK1-silenced or vector-only PC9 GR and NCI-H1975 cells.

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