Fig. 1: USP7 interacts with SAMHD1 at the HD ___domain. | Oncogene

Fig. 1: USP7 interacts with SAMHD1 at the HD ___domain.

From: De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity

Fig. 1

A Flag-tagged SAMHD1 was transfected into the HEK293 cells and immunoprecipitated with an anti-Flag antibody. SAMHD1 was purified with a Flag peptide followed by mass spectrometry analysis. B The lysates of the HCT116 cells were immunoprecipitated with the IgG control or anti-SAMHD1 antibody followed by immunoblotting with USP7 and SAMHD1 antibodies. C The lysates of the HCT116 cells were immunoprecipitated with the IgG control or anti-USP7 antibody followed by immunoblotting with USP7 and SAMHD1 antibodies. D, E SAMHD1 interacts with USP7 in vitro demonstrated by a GST pull-down assay. Recombinant human SAMHD1 or USP7 was incubated with bacterially expressed GST-USP7 or GST-SAMHD1. F SAMHD1 colocalized with USP7 in Hela cells by immunofluorescence assay. Scale bar, 10 μm. G Schematic plot for constructing truncated plasmids of SAMHD1 lacking different domains including NtD (1-34), SAM (35-114), HD (115-583), or CtD (584-626), respectively. H USP7 could not interact with HD ___domain-deleted SAMHD1. Truncated plasmid of SAMHD1 lacking different domains including NtD, SAM, HD, or CtD were constructed for GST pull-down assay with recombinant USP7, respectively. I USP7 interacts with the HD ___domain of SAMHD1.

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