Fig. 1: Expression of S1PR1 in ovarian cancer tissues and paracancerous tissues, various organs, and ovarian cancer cell lines.
From: S1PR1 regulates ovarian cancer cell senescence through the PDK1-LATS1/2-YAP pathway
A Immunohistochemical analysis of S1PR1 in human ovarian cancer tissues (n = 6) and adjacent tissues (n = 2). Scale bar, 200 µm (10 ×); Scale bar, 50 µm (40 ×). B Quantitative analysis of immunohistochemical results. Student’s t-test; ***p < 0.001. C Western blot analysis of S1PR1 in human ovarian cancer tissues (CT) (n = 4) and paracancerous tissues (PT) (n = 2; left) and relative quantitative analysis (right). One-way ANOVA; ***p < 0.001. GAPDH was used as control. D Immunofluorescence analysis of S1PR1 in ovarian cancer cells. DAPI was used to counterstain nuclei (blue). Scale bar, 20 µm. E Western blot analysis of S1PR1 in GC (granular cells derived from normal ovaries), ovarian cancer cell lines (ES-2, A2780, OVCAR-3, HO8910, SKOV3), and IOSE (ovarian cancer epithelial cell line; left) and relative quantitative analysis (right). GAPDH was used as control. One-way ANOVA; ns, p > 0.05; *p < 0.05; ***p < 0.001. F Western blot analysis of S1PR1 in organs (liver, lung, heart, kidney, tummy, skin, ovary, and testicle). GAPDH was used as control.
