Fig. 2: S1PR1 knockout affects the proliferation, migration, and the cell cycle of ovarian cancer cells.
From: S1PR1 regulates ovarian cancer cell senescence through the PDK1-LATS1/2-YAP pathway
A Western blot analysis of S1PR1 expression in wild-type and S1PR1 knockout ovarian cancer cells (A2780 and ES-2). One-way ANOVA; ***p < 0.001. β-actin was used as control. B Cell counting assay was used to detect the proliferation ability of wild-type ovarian cells and S1PR1 knockout cells (A2780 and ES-2). Counting was performed on days 1, 2, and 3 after inoculation and entailed 3 technical replicates per sample. One-way ANOVA; ***p < 0.001. C Soft agar cloning assay was used to compare the proliferation ability of wild-type ovarian cancer cells and S1PR1 knockout cells. Values represent mean ± SD of three independent experiments. One-way ANOVA; *p < 0.05, ***p < 0.001. D Transwell assay analysis of the migration ability of S1PR1 knockout cells (ES-2). Scale bar, 50 µm. Values represent mean ± SD of three independent experiments. Student’s t-test; ***p < 0.001. E Scratch test analysis of the migration ability of S1PR1 knockout cells (ES-2). Scale bar, 50 µm. Values represent mean ± SD of three independent experiments. Student’s t-test; **p < 0.01. F Flow cytometry was employed to analyze the changes in the cell cycle in S1PR1 knockout A2780 and ES-2 cells. Data (each cell line experiment was repeated in triplicate) are presented as mean ± SD.
