Fig. 2: Autophagic flux in 45Ca and p53Rb KO mouse osteosarcoma cell lines.
From: Ligand-dependent hedgehog signaling maintains an undifferentiated, malignant osteosarcoma phenotype
a Western blot analysis of LC3 and ACTIN expression in Hh non-responsive, Trp53 mutant and Rb1 WT CaOS18, CaOS28, and Hh responsive, Trp53 null and Rb1 WT CaOS25 and Trp53 null and Rb1 null C78F, D12M cell lines cultured in 10% serum or serum free media with or without 50 nM bafilomycin-A (Baf-A) for 24 h. n = 5 independent experiments. b Quantification of autophagic flux by western blot in mouse osteosarcoma cell lines as shown in (a). n = 5 independent experiments, mean ± SEM. Graph represents fold change in autophagic flux in serum-free media (induced autophagy) compared to normal serum (basal autophagy) *P < 0.05, one-way ANOVA/Bonferroni correction.
