Fig. 3: MIR31HG modulates stemness-related genes expression in NSCLC cells. | Oncogene

Fig. 3: MIR31HG modulates stemness-related genes expression in NSCLC cells.

From: LncRNA MIR31HG fosters stemness malignant features of non-small cell lung cancer via H3K4me1- and H3K27Ace-mediated GLI2 expression

Fig. 3

A Volcano plot of MIR31HG comparing MIR31HG shv and MIR31HG KD in H1299 cells. B Heatmap showing differentially expressed genes between MIR31HG shv and MIR31HG KD in H1299 cells. C Heatmap showed enriched expression of stemness and Hedgehog related genes in MIR31HG KD group based on the RNA-seq analysis. Color bar indicates gene expression in scale. D Positive correlation between GLI2, SOX2, ABCG2 expression, and MIR31HG expression by generated from TCGA database. E, F The mRNA and protein levels of GLI2, CD133, CD34, ABCG2, and SOX2 upon MIR31HG overexpression or knockdown compared to levels in empty vector detected by RT-qPCR and immunoblotting. G, H The expression of cancer stemness markers CD133 and CD34 were detected by flow cytometry upon MIR31HG overexpression or knockdown compared to levels in empty vector. Data are presented as mean ± SEM; significance abundance: *p < 0.05, **p < 0.01, ***p < 0.001.

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