Fig. 3: UBB suppressed the angiogenesis of RCC cells in vitro. | Oncogene

Fig. 3: UBB suppressed the angiogenesis of RCC cells in vitro.

From: Downregulation of UBB potentiates SP1/VEGFA-dependent angiogenesis in clear cell renal cell carcinoma

Fig. 3

A GO biological processes analysis. B Angiogenesis activity after UBB overexpression was assessed by CAM assay. C Representative capillary tubule structures were observed in HUVEC treated with culture medium from the indicated RCC cells (UBB_NC/UBB_OE). Scale bar, 100 μm. D qPCR analysis of cytokines and growth factors in the process of tumor angiogenesis. E Western blot analysis of VEGFA and PGF in RCC cells (UBB_NC/UBB_OE). F ELISA analysis of VEGFA culture medium from the indicated RCC cells (UBB_NC/UBB_OE). qPCR (G), western blot (H), and ELISA (I) of VEGFA from the indicated RCC cells (UBB_NC/UBB_OE and VEGFA_NC/VEGFA_OE). J Representative capillary tubule structures were observed in HUVEC treated with culture medium from the indicated RCC cells (UBB_NC/UBB_OE and VEGFA_NC/VEGFA_OE). Scale bar, 100 μm. Data are presented as the mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page