Fig. 6: The extracellular MFAP2 activated focal adhesion kinase and regulated motility. | Oncogenesis

Fig. 6: The extracellular MFAP2 activated focal adhesion kinase and regulated motility.

From: MFAP2 is overexpressed in gastric cancer and promotes motility via the MFAP2/integrin α5β1/FAK/ERK pathway

Fig. 6

MFAP2 recombinant protein was added into AGS and SGC-7901 cells. a Cell migration and invasion were evaluated by Transwell assay. b The effect of MFAP2 on FAK/paxillin/ERK tyrosine phosphorylation at different time gradients (390 ng/ml for 0, 5, 10, 15, 20, 25 min). *P < 0.05 vs. control. c The effect of MFAP2 on FAK/paxillin/ERK tyrosine phosphorylation at different concentration gradients (0, 130, 260, 390, 520, 650 ng/ml for 25 min). d, e Western blot and immunostaining showed the effect of MFAP2 on control or si ITGA5/ITGB1 AGS cell focal adhesion formation (×100, Green: F-actin, Red: pY118paxillin or pY397FAK, Blue: hoechst). f Western blot showed the effect of MFAP2 on DMSO or LY3214996-treated AGS.

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