Fig. 2: EWSR1-WT1 directly activates SIK1 expression.
From: Salt-Inducible Kinase 1 is a potential therapeutic target in Desmoplastic Small Round Cell Tumor
A Schematic showing putative E-KTS and E+KTS binding sites in SIK1 2kB proximal promoter and P1-P4 luciferase reporter constructs. B ChIP analysis of SIK1 promoter with IgG or WT1 C-term antibody in BER-DSRCT cells. Areas 1–4 and negative regions were PCR-amplified with the indicated primers (arrows in C). Bands intensities were quantified using ImageJ and normalized to input (n = 3, *p < 0.05, mean ± SEM, student t-test, lower panel). C EWSR1-WT1 directly activates SIK1 promoter. U2OS cells were transfected with pCDNA3-E-KTS, E+KTS or pCDNA3-Empty Vector and P1-P4 reporter constructs. Renilla Luciferase plasmid was used to normalize for transfection efficiency. Relative luciferase activities were calculated relative to pCDNA3-empty vector from 4 independent experiments (mean ± SEM).
