Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Novel insights into stress-induced susceptibility to influenza: corticosterone impacts interferon-β responses by Mfn2-mediated ubiquitin degradation of MAVS

Fig. 4

Stress/CORT-induced viral susceptibility is impaired in MAVS−/− mice. WT and MAVS−/− mice were subjected to restraint stress or CORT treatment. After a 2-d recovery, mice was then administrated nasally with H1N1 virus (2×LD50). CORT (1 mg/kg) dissolved in PEG-400 was administered subcutaneously to mice for 2 d before virus infection. a The survival rate in each group was monitored for 21 d (n = 9). b Viral titers were determined by TCID50 assay at 4 d post infection (n = 4). c MAVS/IFN-β/NP protein expressions were analyzed by western blotting at 4 d post infection. d, e On the 4th day after H1N1 infection, the histological changes and NP protein expression were analyzed by HE and immunohistochemical staining, respectively (n = 3). White arrows indicate the presence of inflammatory infiltrates. Blue arrow indicates hemorrhage exudate. Data are expressed as mean ± SD. *P < 0.05 vs. “WT+H1N1” group; ns, P > 0.05 vs. “MAVS−/−+H1N1” group

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