Fig. 4

Inflammatory response and apoptosis were inhibited in adult mouse cardiomyocytes and H9c2 cell lines by poly(I:C). a Isolated primary cardiomyocytes from adult mice. Blue, DAPI; red, phalloidin-stained microwire skeleton of cardiomyocytes, scale bars, 10 μm. b Expression of inflammatory cytokine CXCL1, CXCL2, IL-1β, and IL-6 as assessed by qRT-PCR in adult mouse cardiomyocytes subjected to OGD with poly(I:C) (n = 3). c, d Representative western blot (c) and average data (d) for Bax, Bcl-2, and cleaved caspase3 in adult mouse cardiomyocytes subjected to OGD with poly(I:C) (n = 3). e, f Representative microphotographs (e) and average data (f) for TUNEL-stained of primary neonatal mouse cardiomyocytes subjected to OGD with 12 h poly(I:C) preconditioning (n = 6). Blue, DAPI; green, TUNEL-positive cardiomyocytes; scale bars, 50 μm. g Expression of inflammatory cytokine CXCL1, CXCL2, IL-1β, and IL-6 in H9c2 cell lines subjected to OGD with poly(I:C) (n = 3). h, i Representative western blot (h) and average data (i) for Bax, Bcl-2 and cleaved caspase3 in H9c2 subjected to OGD with poly(I:C) (n = 3). j, k Representative microphotographs (j) and average data (k) for TUNEL assay of H9c2 cell lines subjected to OGD with 12 h poly(I:C) preconditioning (n = 6) (all experiment groups were compared via one-way ANOVA with a Bonferroni’s multiple comparisons test, bars indicate the SEM, *P < 0.05; **P < 0.01). nOGD, non OGD