Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Extracellular matrix-derived mechanical force governs breast cancer cell stemness and quiescence transition through integrin-DDR signaling

Fig. 5

The biomechanical force promoted tumor cell quiescence through DDR2 signaling. a 3D Matrigel-cultured MCF-7 cells were treated with PBS and integrin β1/3-neutralizing antibodies. Following this, the cell cycle was determined. b Western blotting for DDR1 and DDR2 in MCF-7/4T1/MDA-MB-231 cells cultured in a flask or 3D Matrigel. c DDR2 expression at the mRNA and protein level in 3D Matrigel-cultured MCF-7/4T1/MDA-MB-231 cells treated with scramble or DDR2 siRNA. d Proliferation of 3D Matrigel-cultured MCF-7/4T1/MDA-MB-231 cells treated with scramble or DDR2 siRNA (in 3D Matrigel). e Cell cycle of 3D Matrigel-cultured MCF-7/4T1/MDA-MB-231 cells treated with scramble or DDR2 siRNA. f In vitro colony formation of MCF-7/4T1/MDA-MB-231 cells treated with scramble or DDR2 siRNA. g Western blotting of integrin β1, integrin β3, and AIRE in 3D Matrigel-cultured MCF-7/4T1/MDA-MB-231 cells treated with scramble or DDR2 siRNA. h Western blotting of phosphorylated STAT1, total STAT1, and P27 in flask/3D Matrigel-cultured MCF-7/4T1/MDA-MB-231 cells treated with scramble or DDR2 siRNA. i Schematic diagram of biomechanical force regulating breast cancer cell behaviors through DDRs and integrins signals. Three independent experiments were performed. Data are represented as mean ± SEM. P < 0.05, statistical significance

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