Fig. 2: Binding of baicalin and baicalein with SARS-CoV-2 3CLpro characterized by ITC and ESI-MS. | Acta Pharmacologica Sinica

Fig. 2: Binding of baicalin and baicalein with SARS-CoV-2 3CLpro characterized by ITC and ESI-MS.

From: Anti-SARS-CoV-2 activities in vitro of Shuanghuanglian preparations and bioactive ingredients

Fig. 2

a Representative thermodynamic profiles of baicalin and (b) baicalein binding with SARS-CoV-2 3CLpro in solution, resulting from ITC measurements. c Native-state electrospray ionization mass spectrometry spectra of SARS-CoV-2 3CLpro with baicalin and (d) baicalein. e Binding affinities of baicalin and (f) baicalein with SARS-CoV-2 3CLpro determined by native-state mass spectrometry. Fraction of ligand bound protein ([PL]/[P]t) obtained under optimized conditions plotted against total ligand concentrations. All data are shown as mean ± SD.

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